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Calcification of rat subdermal explants. Calcium was quantified in both 21 day (A) and 90 day (B) explants to determine the effect of the DBP modification and chemistry on the calcium accumulation in BP. (C) Fold change in calcium accumulation from 21 d to 90 d, n=5. *p<0.05 vs control. Control: glutaraldehyde fixed BP, Chemistry: glutaraldehyde fixed BP reacted with EDC and SuOH, DBP: glutaraldehyde fixed BP modified with DBP using EDC and SuOH
