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. Author manuscript; available in PMC: 2016 Jun 14.
Published in final edited form as: Org Biomol Chem. 2015 Jun 14;13(22):6353–6370. doi: 10.1039/c5ob00367a

Table 4.

UPLC-MS Measurements of Relative Ratio of KAUS-12, KAUS-15, and ECA and their Adduct Products with Different Amino Acids

Amino
Acid		 Compound Peak RTa
(min)		 Max.
m/z 		Area Ratio of
Cpd/Adduct
Cysteine Ethacrynic Acid Compound
Adduct		 4.43
2.63		 302.7
423.7		 898
23733		 3.7
96.3
KAUS-12 Compound
Adduct		 2.45
1.79		 368.7
489.7		 19901
9559		 67.5
32.5
KAUS-15 Compound
Adduct		 2.12
1.34		 334.8
455.8		 14150
18915		 42.8
57.2
Lysine Ethacrynic Acid Compound
Adduct		 4.42
N/A		 302.7
N/A		 11524
N/A		 100
N/A
KAUS-12 Compound
Adduct		 2.46
N/A		 368.8
N/A		 21520
N/A		 100
N/A
KAUS-15 Compound
Adduct		 2.13
N/A		 334.9
N/A		 22750
N/A		 100
N/A
Valine Ethacrynic Acid Compound
Adduct		 4.42
N/A		 302.8
N/A		 13128
N/A		 100
N/A
KAUS-12 Compound Adduct 2.46
N/A		 368.8
N/A		 31545
N/A		 100
N/A
KAUS-15 Compound
Adduct		 2.15
N/A		 334.9
N/A		 23461
N/A		 100
N/A
Histidine Ethacrynic Acid Compound
Adduct		 4.42
1.71		 302.8
457.9		 12934
382		 97.1
2.9
KAUS-12 Compound
Adduct		 2.46
N/A		 368.8
N/A		 29432
N/A		 100
N/A
KAUS-15 Compound
Adduct		 2.16
N/A		 334.8
N/A		 17020
N/A		 100
N/A
a

RT = Retention Time