Skip to main content
. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Mol Microbiol. 2015 Apr 6;96(5):1042–1052. doi: 10.1111/mmi.12988

Fig. 5. NADPH and FMN are required for ArsH catalysis.

Fig. 5

PhAs(III) oxidation by PpArsH was assayed with or without NADPH and/or FMN, as described in Materials and Methods. The reaction mixture contained 0.1 µM ArsH, 4 µM PhAs(III), 0.2 mM NADPH and 25 µM FMN. Samples were separated by HPLC using a C4 reverse phase column, and the amount of arsenic in relative counts per second (cps) was estimated by ICP-MS.