FIG. 4.

Inactivation of L. innocua in buffer solution under high-pressure-low-temperature conditions in the frozen phase of water. Treatment parameters: •, 200 MPa, −45°C; ▪, 300 MPa, −45°C (dashed line); ▴, 400 MPa, −45°C (dotted line). Experiments at 300 and 400 MPa included one phase transition from ice I to ice III during compression and back to ice I during decompression. The regression lines and their respective coefficients of determination (R²) were fitted on the basis of the results from the single experiments. Some experiments not shown here were included in the regression analysis.