TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| E. coli | ||
| JM105 | supE endA sbcB15 hsdR4 rpsL thi Δ(lac-proAB) | 25 |
| CH01 | Thioesterase II-negative mutant of E. coli JM105; tesB::Cm(r) | This study |
| S17-1 | recA; harbors the tra genes of plasmid RP4 in the chromosome; proA thi-1 | 26 |
| P. putida GPp104 | PHA synthase-negative mutant of P. putida KT2442 | 10 |
| Plasmids | ||
| pBBR1MCS | Cm(r); broad host range; lacPOZ′ | 16 |
| pBBR1MCS-2 | Kn(r); broad host range; lacPOZ′ | 15 |
| pBluescript SK(−) | Ap(r); lacPOZ′ | 25 |
| pGEM-T | Ap(r);T-vector | Promega Co. |
| pTH19ksl | Kn(r); agp5 lacZ′ repAts1 | 9 |
| pLZZGPp | pBluescript SK(−) derivative, containing phaG gene of P. putida under the control of the lac promoter | 31 |
| pLZZH01 | pGEM-T derivative containing tesB gene of E. coli JM105 | This study |
| pLZZH08 | Deletion of the BamHI-BstBI fragment of plasmid pLZZH01 | This study |
| pLZZH09 | pBBR1MCS-2 derivative, containing tesB gene of E. coli JM105 under the control of the lac promoter | This study |
| pLZZH10 | Deletion of the PmlI—SnaBI fragment of plasmid pLZZGPp | This study |
| pLZZH11 | Insertion of the 1.7-kb BamHI-BstBI fragment of pBBR1MCS into plasmid pLZZH01 | This study |
| pLZZH12 | Insertion of the SacII (filled in)-SacI fragment of pLZZH11 into plasmid pTH19ksl digested with HincII and SacI | This study |