E. coli |
|
|
JM105 |
supE endA sbcB15 hsdR4 rpsL thi Δ(lac-proAB) |
25 |
CH01 |
Thioesterase II-negative mutant of E. coli JM105; tesB::Cm(r) |
This study |
S17-1 |
recA; harbors the tra genes of plasmid RP4 in the chromosome; proA thi-1
|
26 |
P. putida GPp104 |
PHA synthase-negative mutant of P. putida KT2442 |
10 |
Plasmids |
|
|
pBBR1MCS |
Cm(r); broad host range; lacPOZ′ |
16 |
pBBR1MCS-2 |
Kn(r); broad host range; lacPOZ′ |
15 |
pBluescript SK(−) |
Ap(r); lacPOZ′ |
25 |
pGEM-T |
Ap(r);T-vector |
Promega Co. |
pTH19ksl |
Kn(r); agp5 lacZ′ repAts1
|
9 |
pLZZGPp |
pBluescript SK(−) derivative, containing phaG gene of P. putida under the control of the lac promoter |
31 |
pLZZH01 |
pGEM-T derivative containing tesB gene of E. coli JM105 |
This study |
pLZZH08 |
Deletion of the BamHI-BstBI fragment of plasmid pLZZH01 |
This study |
pLZZH09 |
pBBR1MCS-2 derivative, containing tesB gene of E. coli JM105 under the control of the lac promoter |
This study |
pLZZH10 |
Deletion of the PmlI—SnaBI fragment of plasmid pLZZGPp |
This study |
pLZZH11 |
Insertion of the 1.7-kb BamHI-BstBI fragment of pBBR1MCS into plasmid pLZZH01 |
This study |
pLZZH12 |
Insertion of the SacII (filled in)-SacI fragment of pLZZH11 into plasmid pTH19ksl digested with HincII and SacI |
This study |