TABLE 3.

Extracellular accumulation of 3HD by various recombinant E. coli strains^a

E. coli strain	Plasmid	CDW (g/liter)	3HD (mg/liter)	%3HD/CDW (wt/wt)
JM105	pBluescript SK(−), pBBR1MCS-2	1.84 ± 0.05	NDb
	pBluescript SK (−), pLZZH09	1.75 ± 0.03	ND
	pLZZGPp, pBBR1MCS-2	2.11 ± 0.04	642 ± 12	30.4 ± 0.5
	pLZZGPp, pLZZH09	2.20 ± 0.04	1,021 ± 22	45.9 ± 0.8
CH01	pBluescript SK(−), pBBR1MCS-2	1.81 ± 0.03	ND
	pLZZGPp, pBBR1MCS-2	2.10 ± 0.05	72 ± 6	3.4 ± 0.1
	pLZZGPp, pLZZH09	2.21 ± 0.03	956 ± 18	43.3 ± 0.6

^aRecombinant E. coli strains were cultivated in 500-ml conical flasks containing 100 ml of Luria-Bertain medium supplemented with 100 mg of ampicillin/liter and 50 mg of kanamycin/liter according to the resistance properties of the plasmids (Table 1 and Fig. 1). E. coli CH01 is the isogenic tesB-negative knockout mutant of E. coli JM105. Plasmid pLZZGPp is a derivative of the vector pBluescript SK(−) harboring phaG under the control of the lac promoter, while pLZZH09 is a derivative of the vector pBBR1MCS-2 harboring tesB under the control of the lac promoter. Cells were grown at 37°C and 200 rpm for 48 h; 1 mmol of IPTG/liter, 20 g of fructose/liter, and 0.1 mg of triclosan/liter were added to the culture after 9, 12, and 24 h, respectively. The data are shown as means ± SEM (n = 6).

^bND, not detectable.