TABLE 1.
Comparative stoichiometries of reactants and products during biotransformation of CL-20 by the salicylate 1-monooxygenase from Pseudomonas sp. strain ATCC 29352 and the membrane-associated enzyme(s) from Pseudomonas sp. strain FA1
| Reactant or product |
Pseudomonas sp. strain ATCC 29352a,c
|
Pseudomonas sp. strain FA1b,c
|
||
|---|---|---|---|---|
| Amt (nmol) | Molar ratio of reactant to product per reacted CL-20 molecule | Amt (nmol) | Molar ratio of reactant to product per reacted CL-20 molecule | |
| Reactants | ||||
| CL-20 | 25 ± 1.4 | 1.0 ± 0.05 | 20 ± 1.2 | 1.0 ± 0.06 |
| NADH | 48 ± 2.7 | 1.9 ± 0.10 | 90 ± 7.1 | 4.5 ± 0.35 |
| Products | ||||
| Nitrite (NO2−) | 43 ± 2.5 | 1.7 ± 0.09 | 46 ± 3.2 | 2.3 ± 0.16 |
| Nitrous oxide (N2O) | 79 ± 5.3 | 3.2 ± 0.21 | 29 ± 1.6 | 1.5 ± 0.08 |
| Formate (HCOOH) | 37 ± 2.4 | 1.5 ± 0.09 | 34 ± 2.8 | 1.7 ± 0.13 |
| Ammonium (NH4+) | 15 ± 0.9 | 0.6 ± 0.04 | NDd | ND |
a
Reaction was performed at pH 7.0 and 30°C for 2 h under anaerobic conditions.
b
Data are from reference 4.
c
Data are mean ± standard error (n = 3).
d
ND, not determined.