Skip to main content
. 2015 Apr 27;29(6):842–855. doi: 10.1210/me.2014-1279

Figure 3.

Figure 3.

Knockdown of endogenous Six6 and Six3 mRNAs in gonadotrope cell lines affects gonadotrope-specific mRNA expression. A, LβT2 cells were transfected with SMARTpools of nontargeting or Six6 siRNA for either 48 or 72 hours. B, αT3-1 cells were transfected with SMARTpools of nontargeting or Six3 siRNA for either 48 or 72 hours. All values represent the SQ ± means from 4 independent experiments with all means adjusted to corresponding GAPDH values within experiment, using relative standard curve for analysis. Statistical analysis by one-way ANOVA (GraphPad Prism 5). *, P < .05; ***, P < .001 as compared with nontargeting siRNA.