TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference |
|---|---|---|
| E. coli TOP10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| H. influenzae | ||
| Rd KW20 | WT genome sequence strain | 19 |
| SM1 | Rd(ΔribC::Kan) Kanr | This study |
| ASK1 | Rd(ΔompP1::cat) Camr | This study |
| ASK2 | Rd(ΔompP1::tetA tetR) Tetr | This study |
| ASK3 | Rd(ΔompP1::ribC+cat) Camr | This study |
| ASK4 | Rd(ΔompP1::ribC+tetA tetR) Tetr | This study |
| ASK5 | Rd(ΔribC::Kan ΔompP1::ribC+cat) Camr Kanr | This study |
| SM2 | Rd(ΔribC::Kan ΔompP1::ribC+tetA tetR) Tetr Kanr | This study |
| Plasmids | ||
| pUC18K | Kmr Apr; pUC18 containing nonpolar aph-A3 cassette | 8 |
| pUC19 | Apr; cloning vector | 20 |
| pGEM-T | Apr; TA cloning vector | Promega |
| pCMR | Cmr; 1.3-kb Cmr cassette from pACYC184 in pCR2.1 | 18 |
| pGESYII | Tcr; 2.8-kb Tcr cassette from pGJB103 in pACYC177 | 14 |
| pSM1 | Apr; pGEM-T 2,039-bp DNA fragment containing ribC and flanking region | This study |
| pSM2 | Kmr Apr; pSM1 with 442-bp deletion in ribC replaced by aph-A3 | This study |
| pSM3 | Apr Tcr; pGEM-T::tetA tetR | This study |
| pASK1 | Apr; pGEM-T, 645-bp ompP1 5′ homology sequence | This study |
| pASK2 | Apr; pGEM-T, 606-bp ompP1 3′ homology sequence | This study |
| pASK3 | Apr; pUC19 with ompP1 5′ 3′ homology sequence | This study |
| pASK4 | Apr Cmr; pGEM-T, 1238-bp Cmr cassette from pCMR flanked by SmaI, BamHI, XbaI, SalI, and PstI sites | This study |
| pASK5 | Apr Cmr; pUC19, ompP1 5′, 3′ homology sequence | This study |
| pASK6 | Apr Tcr; pUC19, ompP1 5′, 3′ homology sequence | This study |
| pASK7 | Apr; pGEM-T::ribC | This study |
| pASK8 | Apr Cmr; pUC19, ompP1 5′, 3′ homology sequence; ribC | This study |
| pASK9 | Apr Tcr; pUC19, ompP1 5′, 3′ homology sequence; ribC | This study |