E. coli TOP10 |
F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (Strr) endA1 nupG
|
Invitrogen |
H. influenzae |
|
|
Rd KW20 |
WT genome sequence strain |
19 |
SM1 |
Rd(ΔribC::Kan) Kanr
|
This study |
ASK1 |
Rd(ΔompP1::cat) Camr
|
This study |
ASK2 |
Rd(ΔompP1::tetA tetR) Tetr
|
This study |
ASK3 |
Rd(ΔompP1::ribC+cat) Camr
|
This study |
ASK4 |
Rd(ΔompP1::ribC+tetA tetR) Tetr
|
This study |
ASK5 |
Rd(ΔribC::Kan ΔompP1::ribC+cat) Camr Kanr
|
This study |
SM2 |
Rd(ΔribC::Kan ΔompP1::ribC+tetA tetR) Tetr Kanr
|
This study |
Plasmids |
|
|
pUC18K |
Kmr Apr; pUC18 containing nonpolar aph-A3 cassette |
8 |
pUC19 |
Apr; cloning vector |
20 |
pGEM-T |
Apr; TA cloning vector |
Promega |
pCMR |
Cmr; 1.3-kb Cmr cassette from pACYC184 in pCR2.1 |
18 |
pGESYII |
Tcr; 2.8-kb Tcr cassette from pGJB103 in pACYC177 |
14 |
pSM1 |
Apr; pGEM-T 2,039-bp DNA fragment containing ribC and flanking region |
This study |
pSM2 |
Kmr Apr; pSM1 with 442-bp deletion in ribC replaced by aph-A3
|
This study |
pSM3 |
Apr Tcr; pGEM-T::tetA tetR
|
This study |
pASK1 |
Apr; pGEM-T, 645-bp ompP1 5′ homology sequence |
This study |
pASK2 |
Apr; pGEM-T, 606-bp ompP1 3′ homology sequence |
This study |
pASK3 |
Apr; pUC19 with ompP1 5′ 3′ homology sequence |
This study |
pASK4 |
Apr Cmr; pGEM-T, 1238-bp Cmr cassette from pCMR flanked by SmaI, BamHI, XbaI, SalI, and PstI sites |
This study |
pASK5 |
Apr Cmr; pUC19, ompP1 5′, 3′ homology sequence |
This study |
pASK6 |
Apr Tcr; pUC19, ompP1 5′, 3′ homology sequence |
This study |
pASK7 |
Apr; pGEM-T::ribC
|
This study |
pASK8 |
Apr Cmr; pUC19, ompP1 5′, 3′ homology sequence; ribC
|
This study |
pASK9 |
Apr Tcr; pUC19, ompP1 5′, 3′ homology sequence; ribC
|
This study |