TABLE 3.
Transformation efficiencya of ribC disruption constructs
| DNA constructb | With riboflavinc | Without riboflavind | Ratioe |
|---|---|---|---|
| Plasmid DNA (pSM2) | 4.9 × 105 | 7.1 × 103 | 69 |
| Linear DNA | 2.2 × 106 | 1 × 102 | 22,000 |
a
Transformation efficiency refers to the number of kanamycin-resistant colonies recovered per microgram of DNA. These data represent a single representative (reproducible) experiment.
b
ribC disruption constructs as referred to in Materials and Methods and Fig. 1b.
c
Number of kanamycin-resistant colonies recovered when selection is carried out in the presence of 100 μg of riboflavin per ml.
d
Number of kanamycin-resistant colonies recovered when selection is carried out in the absence of riboflavin.
e
Ratio refers to the number of kanamycin-resistant colonies obtained in the presence of riboflavin divided by the number of kanamycin-resistant colonies obtained in the absence of riboflavin.