PCR amplification of partial gyrA sequences from F. psychrophilum isolates. Lanes M, molecular size markers (HyperLadder 1; Bioline, London, United Kingdom); lanes 1, 2, and 3, PCR products of the F. psychrophilum type strain (NCIMB1947), FPC814, and FPC840, respectively. (A) Amplification from F. psychrophilum isolates with degenerate universal primers gyrAF and gyrAR. (B) Amplification of the predicted 396-bp fragments (including primers) from F. psychrophilum isolates with specific primers GYRA-FP1F and GYRA-FP1R.