. 2004 Jul;70(7):3979–3987. doi: 10.1128/AEM.70.7.3979-3987.2004

TABLE 1.

Characterization and classification based on rRNA ITS DGGE of isolated Microcystis colonies^a

Colony type	ITSa group	ITSc group	Colony K:	Microcystis morphospecies^b	Lake^c
Toxin producing	1	5	21*	W	J
			69*	B	Z
			107	B	K
			146, 147-u	A	W
	1	18	147-l	A	W
	2	4	63-u	B	Z
	4	1	79-u	I/P	Br
	4	2	79-l	I/P	Br
	4	15	112-u^e	I	M
	6	4	15-u, 19-m	B	J
	6	5	63-l	B	Z
	8	5	12, 15-l,19-l	B	J
	11	5	3-u	N	J
			10-u	A	J
			42-u, 43-u, 59	B	Z
			41-u, 46, 47-u, 49,50, 52, 55-u, 57, 58	A	Z
			83	A	Br
			102	A	MI
			111	A	M
			139-u	P	A
			143	B	W
			144	A	W
	11	8	51, 72	B	Z
	11	10	3-l	N	J
			41-l,47-l,55-l	A	Z
			42-l, 43-l	B	Z
			139-l	P	A
	15	15	112-l^e	I	M
	19	12	60-u	A	Z
	23	3	114-u	F	M
	23	14	10-l	A	J
			114-l	F	M
	26	8	9, 14, 20	A	J
	26	12	13^e	A	J
			60-l	A	Z
	27	19	39	A	J
Unknown toxin production	1	4	65*	A	Z
Unknown toxin production	7	13	29*	A	J
	10	10	61-u, 70-m, 66*	A	Z
	13	17	32*	I	J
	17	5	67-u*	I	Z
	17	11	61-l*	A	Z
	17	17	67-l*	I	Z
	17		70-l*	A	Z
	21	18	31-l*	A	J
Non-toxin producing	2	11	75-u	A	Ba
Non-toxin producing	145	I	W
	3	16	75-l	A	Ba
			142	I	W
	5	ND	119-u, 120-u	I/P	Pw
	6	14	128, 129	A	R
	9	16	106-u	I	H
	9	ND	119-m, 120-l	I/P	Pw
	10	6	5-u^d	N	J
	11	14	17-m	I	J
	11	15	99-u, 150	I	MI
	11	16	17-l	I	J
			97	F	MI
			99-l, 101, 149	I	MI
			140	P	A
	12	1	130	I	R
			151*	A	R
	12	9	73, 76	I	Ba
			132, 134	I	R
	12	11	18	A	J
	14	11	16^d	W	J
	14	16	106-l	I	H
	16	9	108, 110	I	T
			117	I	Pc
			121	P	Pw
			123	I/P	Pw
	16	ND	119-l	P	Pw
	17	6	74	A	Ba
			131	W	R
			133	A	R
	17	9	5-l^d	N	J
			127	I	R
	18	1	78-u	A	Bo
	18	2	78-l	A	Bo
	18	7	148	I	T
	18	17	105-u	I	Pe
	18	18	87, 88, 89, 90, 91, 92, 95	A	F
			93, 94	I	F
			100^e	I	MI
	20	7	33-u	I	J
	21	5	135, 136	A	A
			137, 138, 141	P	A
	22	13	34, 35, 36	I	J
			38^*	W	J
	24	17	37	I	J
			68	I	Z
			105-l	I	Pe
			31-u*	A	J
	25	3	104-u	I	H
	25	11	33-l	I	J
	25	14	104-l	I	H

Morphospecies assignment and lake of origin are given for each colony. Based on the positions of their bands in the ITSa and ITSe DGGE profiles, colonies K1 to K151 were assigned group numbers. Classes of colonies were distinguished on the basis of combined ITSa and ITSc grouping. Colonies in boldface type contained microcystins as determined by MALDI-TOF MS or, in a few cases, by amplification of the mcy gene. Toxin production data were lacking for colonies indicated by asterisks. Colony classes were organized in three groups: one with classes containing toxin-producing colonies, one with nontoxic colony classes, and one with classes of colonies of unknown toxin production. The few colonies of unknown toxin production that classified with toxic or nontoxic colonies ended up in the group of either. Bands of underlined colonies were sequenced. The suffix “-u” refers to the upper band in the ITSc DGGE profile from a colony, the suffix “-m” refers to the middle band, and the suffix “-l” refers to the lower band. If the upper and lower bands from a particular colony have the same group assignment for, e.g., ITSa, this means that there is one band in the ITSa profile and two bands in the ITSc profile. Note that if a mixture of 2 sequences gave rise to 2 ITSa and 2 ITSc bands, their relative band positions (meaning upper or lower) may be reversed. ND, not determined.

A, M. aeruginosa; B, M. botrys; I, M. ichthyoblabe; F, M. flos-aquae; N, M. novacekii; P, M. panniformis; W, M. wesenbergii.

Lakes: A, Lake Arancio, Sicily, Italy; Ba, Loch Balgavies, Dundee, Scotland; Bo, Lac du Jardin Public, Bordeaux, France; Br, Brno reservoir, Brno, Czech Republic, F, lake Frederiksborg Sløtssø, Hillerød, Denmark; H, Holzöstersee, Salzburg, Austria; J, ’t Joppe, Leiden, The Netherlands; K, Krossinsee, Berlin, Germany; M, Mügelsee, Berlin, Germany; MI, Monikie Island Pond, Dundee, Scotland; T, Lake Takerkoust, Marrakesh, Morocco; Pe, Parque de Cicade pond east, Porto, Portugal; Pw, Parque de Cicade pond west, Porto, Portugal; R, Loch Rescoby, Dundee, Scotland; W, Wannsee, Berlin, Germany; Z, Zeegerplas, Alphen aan de Rijn, The Netherlands.

Toxin production was examined by mcy gene detection only (no MALDI-TOF MS).

The MALDI-TOF MS profile showed only 1 peak at a mass-to-charge ratio (m/z) of 995, which was not analyzed by PSD measurements and could therefore be derived from either microcystin LR or cyanopeptolin.