TABLE 3.
Comparison of sequences derived from DGGE gels from natural samples with sequences derived from isolated coloniesa
| ITSa band sequence(s) | ITSc band sequence(s) | Matching colony(ies) |
|---|---|---|
| e3 | e28 | K46, K49, K51b (+) |
| e4, e5, e9 | e21, e22 | K74, K131 (−) |
| e6, e11 | K148, K105-u (−) | |
| e8 | K16 (−) | |
| e12, e15 | K34 (−) | |
| e17 | K17, K99, K150 (−) | |
| e25, e30 | K37, K68b (−) | |
| e2, e7, e13, e16 | e24, e31 | None |
| e10 | e26 | None |
| e14 | e29 | None |
| e23 | None |
a
DGGE gels from natural samples are shown in Fig. 3. Each row contains identical sequences (ITSa sequences were compared with the 5′ region of the longer ITSc fragments). In the last column, colonies with sequences identical to those of excised bands are presented. Toxin production in these colonies is indicated as follows: (+), toxic; (−), nontoxic.
b
Not an exhaustive list; there were more colonies for which the sequences matched equally well.