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. 2004 Jul;70(7):3839–3844. doi: 10.1128/AEM.70.7.3839-3844.2004

FIG. 1.

FIG. 1.

Overproduction of Mn-dependent catalases from T. thermophilus HB8 in E. coli. (A and C) Overproduction at 37°C (A) or 22°C (C) of His-tagged Cat and Cat* proteins from pET28b derivatives (1 mM IPTG). (B and D) Overproduction at 37°C (B) or 22°C (D) of unmodified Cat and Cat* proteins from pET22b derivatives (1 mM IPTG). Lane 1, insoluble fractions of cells harboring pET22b or pET28b (control); lane 2, insoluble fractions of cells harboring the cat* gene; lane 3, insoluble fractions of cells harboring the cat gene; lane 4, soluble thermostable (70°C for 10 min) fractions of cells harboring pET22 or pET28b (control); lane 5, soluble thermostable fractions of cells expressing the cat* mutant gene; lane 6, soluble thermostable fractions of cells expressing the wild-type cat gene; lane M, molecular weight markers.