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Overproduction of Mn-dependent catalases in T. thermophilus. Lane 1, molecular mass markers (94, 67, 43, 30, and 20.1 kDa); lanes 2 and 3, soluble extract from T. thermophilus HB8 harboring pMKE1 (control) or pMKEcat; lanes 4 to 6, soluble extracts from T. thermophilus HB27::nar harboring pMKE1 (control), pMKEcat*, and pMKEcat, respectively. The position corresponding to the overexpressed Mn-dependent catalase is indicated by an arrow.
