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Thermostability of the Cat and Cat* proteins. Thermal inactivation of glyoxyl derivatives of the unmodified wild-type Cat (▴) and mutant Cat* (⧫) Mn-dependent catalases overproduced in T. thermophilus HB27::nar was determined. The experimental conditions were as follows: 85°C and 25 mM potassium phosphate buffer (pH 7.0). The amount of incubated enzyme was 25 IU/ml.
