TABLE 2.
Novel oligonucleotide probes targeting prasinophycean taxa
| Probe name | Sequence | Target group | Position of 16S rRNA (Escherichia coli) | Closest sequence not targeted
|
|
|---|---|---|---|---|---|
| Taxonomy | No. of mismatches | ||||
| PRAS01 | 5′-ACG GTC CCG AAG GGT TGG-3′ | Pseudoscourfieldiales clade V | 193 | Tilletia caries | 2 |
| PRAS03 | 5′-GCC ACC AGT GCA CAC CGG-3′ | Prasinococcales | 620 | Friedmannia israeliensis | 2 |
| PRAS04 | 5′-CGT AAG CCC GCT TTG AAC-3′ | Mamiellales (except the genus Dolichomatix) | 651 | Choricystis minor | 1 |
| PRAS05 | 5′-GCC AGA ACC ACG TCC TCG-3′ | Clade VIIA, RCC 287, CCMP 1205 | 651 | Pyramimonas olivacea | 3 |
| PRAS06 | 5′-AAT CAA GAC GGA GCG CGT-3′ | Environmental clade VIIB | 651 | Scutopus ventrolineatus | 3 |
| MICRO01 | 5′-AAT GGA ACA CCG CCG GCG-3′ | M. pusilla | 211 | Ostreococcus tauri | 1 |
| BATHY01 | 5′-ACT CCA TGT CTC AGC GTT-3′ | B. prasinos | 651 | Uncultivated bacteria | 3 |
| OSTREO01 | 5′-CCT CCT CAC CAG GAA GCU-3′ | Ostreococcus | 647 | Corynebacterium genitalium | 3 |