TABLE 3.

Primers and oligonucleotide probes used in study 2^a

Target	Primer	Nucleotide sequence (5′ to 3′)	Temp (°C)
prs-hly	PRSF	TTACAAAYTCCATYGCTCTTCCAGAAGAMAAATGGA	60-63
	T7-ISOR	TAATACGACTCACTATAGGGAACCGTTCTCCACCATTCCCA	68
hly-mpl	ISOF	GTTAATGAACCTACAAGHCCTTCCb	54-56
	T7-MPL-LMR	TAATACGACTCACTATAGGGAGTGGAAGCGATTCGTAATCTTTCATATGAG	68
mpl-plcB	MPL-LMF	CTCATATGAAAGATTACGAATCGCTTCCACTC	61
	T7-PLCBR	TAATACGACTCACTATAGGGTCATCTGAGCAAAATCTTTTTGCTACCATGTC	68
actA-ldh	ActA2F	CTAGCTGATTTAAGAGATAGAGGAACAG	57
	T7-LDHR	TAATACGACTCACTATAGGGTTGTTGAAATGAACTTAAAYGACAAAGAAAA AGAACAAATGAA	66-67
gcaD	GcaDF	CATGGGAGATGCGGAAATTGGTAAAAAC	58
ctc	Ctc-R	GGAAGATATCGCAAACAAATGCGAATC	57
lin0372	Lin0372F	CGTTGTTTTGGCGTTGTCGATTGTTATTG	59
	Lin0372R	TACACGATGATCTCCTGTTGCTGGTAG	60
Plasmid pLI100	LinPNCR1F	GTACATCATGCAAACGATTTTCTCAAGTATC	58
	LinPNCR1R	CTGTCTACTTTATTGACATCTATCCAACCT	58
	Lin0198F	ATGAACAAATTAGTTAGTCAAAGTAATG	51
lin0198	Lin0198R	TATCGATGTCTTGAGGTCACACAAAGTTC	59
	Lin2454F	TGGAAGTACTTATACGCCAGGAATTATTC	57
lin2454	Lin2454R	CAAAATGCTTTATTTTCAATAATCATTTCATC	53
	Lin0419F	CATTAAATTAGCTCTAAAACAACTACTATC	53
lin0419	Lin0419R	TACACCAAACACAATTCCAATAAACATC	54
lin0454-lin0455	LinNCR1F	GGATTTGGTAAATTATACAAAGGTTTTAAG	53
	LinNCR1R	TGCTTCTTGGCATTTTAGTAATCTTTC	54
nadA-lin2134	LinNCR3F	TGCGTTAAAAGCATTAGAAAGAATGCTC	56
	LinNCR3R	AGTGAAGGTATTATCGACTGGTGGTAG	58
prfA	prfAR	GTATTTTTCTATATGATGGTATCACAAAGCTC	57
	prfAF	CATATCTTTTGAGATAATCAAGATTTTGTAC	54
plcA	PlcAF	AAGTTGAGTACGAAYTGCTCTACTTTGTTG	58-59
	PlcAR	AACACAAACGATGTCATTGTACCAACAACTAG	59
actA	ActAR	GCTTATTTTCGATACCTTTGGACTTAACGAAG	59
plcB	PlcBF	GATAACCCGACAAATACTGACGTAAATAC	57
prfA	PrfA1probe	CATATCTTTTGAGATAATCAAGATTTTGTACAT	54
	PrfA2probe	TAAATTATCCAGTGTAATCTTGATGCCATC	56
	PrfA3probe	ACTCGTGAGCTTTGTGATACCATCATAT	57
plcA	PlcA1probe	TACTGACGAGGTGTGAATGTTAATGAAGT	57
	PlcA2probe	TCGTCTTTTAAACGCATAATAATGGTTTCTTTTGG	59
	PlcA3probe	ACATCCATTGTTTTGTAGTTACAGAGTTCTT	56
hly	HlyM1probe	ACTGGAGCGAAAACAATAAAAGCAAGCTAGC	60
	HlyM2probe	AACTCAGAATATATTGAAACAACTTCAAAAG	54
	HlyM3probe	ATGATCCTGAAGGTAACGAAATTGTTCAAC	58
mpl	Mpl1probe	ATTCTTGATATGACAAAAGATAAGCTAGGC	56
	Mpl2probe	ACGCTTAGTTTATAATATAAAATTGATTTTTG	52
	Mpl3probe	TGATTCGTTTGTCCATTATGGCTTGAATTG	58
actA	ActA1probe	ATATTTGCAGCGACAGATAGCGAAGATTC	59
	ActA2probe	GATAGTGAGCTTGAAAGCCTTACTTATCC	59
	ActA3probe	AGTAAAGAAAGCGATTGTTGATAAAAGTGC	56
plcB	PlcB1probe	AGTACATTTTTATCTCATTTTTATAATCCTGATAG	55
	PlcB2probe	ATGCGGATCATAAAAATCCATATTATGATACT	55

^aUnderlined sequences are the T7 RNA polymerase promoter sequences.

^bH is A, C, or T (due to ambiguity).