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. 2015 Mar 26;125(22):3377–3387. doi: 10.1182/blood-2015-01-620278

Figure 6.

Figure 6

Mechanism of hyperglycemia-mediated downregulation of miR-24. (A) Western blot analysis of VWF and c-Myc expression in cell lysates under conditions of normal or high glucose (72-hour cultured HUVECs) in the presence or absence of an aldose reductase inhibitor (epalrestat). (B) qPCR quantitation of miR-24 expression in normal or high glucose (72-hour cultured HUVECs) with or without aldose reductase inhibitor (epalrestat); **P < .01; *P < .05. (C) Time course analysis of c-Myc mRNA expression in normal or high glucose (cultured HUVECs) using qPCR quantitation (normalized to GAPDH). (D) Endothelial cells were incubated with different concentrations of H2O2 (0 to 2000 μM) for 1 hour prior to analysis of cell lysates with western blot. (E) Western blot analysis of endothelial cell lysates when transfected with si-cMyc or si-Control followed by qPCR quantitation of miR-23/24/27 clusters expression; ***P < .001; **P < .01. (F) Western blot analysis of endothelial cell lysates when transfected with si-cMyc or si-Control followed by qPCR quantitation of miR-24/335/503 expression; ***P < .001; **P < .01.