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. Author manuscript; available in PMC: 2015 May 29.
Published in final edited form as: Brain Behav Immun. 2013 Jun 28;33:112–122. doi: 10.1016/j.bbi.2013.06.004

Figure 5.

Figure 5

TNFα release from neonatal microglia (b) and neonatal astrocytes (c) incubated with LPS (100 ng/ml) for 24 h was attenuated by ATL313 (1 μM). Administration of H-89 (PKA inhibitor) partially reversed the effects of ATL313 on TNFα production in microglia (A) but not astrocytes (B). IL-10 production induced by LPS was not affected by ATL313 or H-89 in microglia (C, D). Administration of chelerythrine (PKC inhibitor) had no effect on ATL313 mediated effects of TNFα production in microglia (E) but reversed the ATL313 effect in astrocytes (F). IL-10 production induced by LPS was not affected by ATL313 in microglia (G). However, chelerythrine+LPS+ATl313 increased IL-10 compared to LPS+ATL313+vehicle in microglia (G). Chelerythrine had no effect on IL-10 responses in astrocytes (H). Protein measured by rat-specific ELISA. *P<0.05, **P<0.01, ***P<0.001 compared to LPS+ATL313+veh; n=4-5 wells/group. All data are mean±SEM.