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. Author manuscript; available in PMC: 2015 May 29.
Published in final edited form as: Phys Chem Chem Phys. 2015 Apr 29;17(18):11933–11949. doi: 10.1039/c4cp05286b

Fig. 11.

Fig. 11

Quantum yields and various reactions times of four elementary steps for wild type (EcCPD) and the two mutants.32 (A) The overall repair quantum yields (QY) of the two mutants N378C and E274A were measured, relative to the known wild-type one (0.82), by monitoring the formation of thymine bases at 266-nm absorption with certain visible-light irradiation of the enzyme-substrate solution. (B) The reaction times of each elementary step in CPD repair by the wild type and the two mutants with our measured total QY from (A). The vertical dashed lines represent the two-step repair efficiency of the FET to lifetime emission (LT) and the second-bond splitting (SP2) to BET. The ER is decoupled from the CPD splitting.