Table 2.

ropB Alleles Identified in 1178 Serotype M3 GAS Strains

Allele No.	Codon	Nucleotide Change	Amino Acid Change	Occurrences, No.	Position in Codon	Strain Collections	SpeB Expression and Activity	Strains Tested for SpeB, No.a
1	Wild-type	None	None	852	…	All	Wild-typeb	9
2	1	G3A	M1I	1	3	OI	Absent	1
3	1	T2A	M1K	1	2	US	Absent	1
4	6	A16C	T6P	1	1	OI	Reduced	1
5	7	G19A	V7I	104	1	OI, US, UK, OP	Reducedc	56
6	7, 61	G19A, T182C	V7I L61P	1	2	OP	Absent	1
7	7, 103	G19A, T307C	V7I S103Pd	1	1	OP	Absent	1
8	7, 104	G19A, C311T	V7I T104I	1	2	OP	Absent	1
9	7, 226	G19A, G677A	V7I R226Qd	47	2	OI, OP	Absente	28
10	7, 235	G19A, T704C	V7I I235T	1	2	US	Absent	1
11	11	G33-1	FS/truncation	1	1	US	Absent	1
12	22	G65A	C22Y	8	2	OI, US, OP	Absent	7
13	28	G83A	R28K	1	2	UK	Absent	1
14	28	A82G	R28G	1	1	GDR	Absent	1
15	29	C85G	Q29E	1	1	GDR	Absent	1
16	31	A92G	Y31C	1	2	US	Absent	1
17	33	C97 + 18	6 AA Insertion	4	In frame	US	Reduced	2
18	33	C97A	R33S	1	1	AP	Reduced	1
19	34	T100A	F34I	1	1	OI	Absent	1
20	43	C128A	Nonsense	1	2	OI	Absent	1
21	55	T164A	V55D	1	2	US	Absent	1
22	57	G169A	V57I	3	1	US	Wild-type	3
23	58	G172A	D58N	1	1	AP	Absent	1
24	62	T186 + 1	FS/truncation	7	1	AP	Absentf	7
25	63	T188C	I63T	12	2	US	Absent	2
26	68	A204C	K68N	2	3	US	Wild-type	1
27	72	G214T	Nonsense	1	1	US	Absent	1
28	73	T218G	F73C	1	2	US	Absent	1
29	75	G223A	D75N	1	1	US	Wild-type	1
30	76	G228A	M76I	1	3	US	Absent	1
31	80	A240C	K80N	14	3	US, OP	Absent	2
32	83	T248C	F83S	1	2	OI	Absent	1
33	85	G254A	C85Y	11	2	OI, US	Reduced	4
34	87	C261 + 1	FS/truncation	2	1	US	Absent	2
35	90	G268A	G90D	1	1	US	Absent	1
36	91	T272C	L91S	1	2	US	Absent	1
37	94	T281A	I94N	1	2	OI	Absent	1
38	100	G298 + 20	FS/truncation	1	2	US	Absent	1
39	103	T307C	S103P	5	1	OI, reference	Reduced	2
40	103	C308A	Nonsense	1	2	US	Absent	1
41	105	A314T	K105M	1	2	US	Absent	1
42	107	G321T	K107N	3	3	US	Wild-type	3
43	111	G331A	A111T	3	1	US	Absent	2
44	111	C332A	A111D	1	2	UK	Absent	1
45	134	C402-8	FS/truncation	1	1	US	Absent	1
46	136	C406A	L136I	1	1	US	Reduced	1
47	140	G418-1	FS/truncation	4	2	OP	Absent	4
48	140	G418 + 6	2 AA insertion	1	In frame	US	Absent	1
49	142	G425A	Nonsense	1	2	US	Absent	1
50	143	G428A	S143N	1	2	OI	Absent	1
51	150	T448A	F150I	1	3	US	Absent	1
52	151	A451T	N151Y	3	1	OI	Absent	2
53	151	T453A	N151K	1	3	US	Absent	1
54	152	A455T	N152I	1	3	US	Absent	1
55	154	G462A	M154I	1	3	OI	Absent	1
56	173	C517T	L173F	1	1	US	Absent	1
57	179	T536A	L179Q	1	2	US	Absent	1
58	180	G539A	R180K	1	2	GDR	Absent	1
59	184	T552A	N184K	2	3	OI	Absent	2
60	189	G567A	M189I	3	3	OI, US	Absent	2
61	196	T587G	L196W	2	2	UK	Absent	2
62	202	A605G	E202G	1	2	US	Absent	1
63	206	C617A	A206D	1	2	OI	Absent	1
64	206	C617-1	FS/truncation	1	3	UK	Absent	1
65	220	G658T	D220Y	3	1	UK	Absent	2
66	222	G665A	C222Y	4	2	OI, AP	Absent	4
67	224	T670C	Y224H	4	1	OI	Absent	2
68	226	G677A	R226Q	1	2	US	Absent	1
69	227	G680A	C227Y	1	2	OI	Absent	1
70	232	T696 + 1	Nonsense	2	1	OI, UK	Absent	1
71	232	T696-1	FS/truncation	6	1	US, GDR	Absent	2
72	237	G710T	G237V	1	2	OI	Absent	1
73	238	T713C	L238P	2	2	OP	Absent	2
74	245	G733A	A245T	2	1	OI, GDR	Absent	2
75	245	G733C	A245P	2	1	US	Absent	2
76	247	C739A	Nonsense	4	1	OI	Absent	2
77	249	G746A	C249Y	2	2	US	Absent	2
78	252	A754T	I252F	1	1	OI	Absent	1
79	252	C756-14	FS/truncation	2	1	US	Absent	2
80	256	T767C	F256S	1	2	US	Absent	1
81	260	A779C	N260T	1	2	UK	Wild-type	1
82	267	G801A	M267I	2	3	OI, US	Absent	2
83	268	T803G	F268C	1	2	OI	Absent	1
84	271	T811C	Y271H	1	1	AP	Reduced	1

Abbreviations: AA, amino acid; AP, Alberta pharyngitis strains; GDR, German Democratic Republic invasive strains; OI, Ontario invasive strains; OP, Ontario pharyngitis strains; SpeB, secreted streptococcal cysteine protease B; UK, United Kingdom invasive strains; US, United States Centers for Disease Control and Prevention invasive strains.

^aRandomly selected invasive strains and all pharyngitis strains with a regulator of protease B gene (ropB) polymorphism were tested for SpeB.

^bAll strains tested secreted high levels of SpeB.

^cOf 56 strains tested, 48 secreted SpeB (10 at near wild-type levels, 38 at reduced levels; 8 lacked detectable SpeB).

^dThe second single-nucleotide polymorphism occurring in the V7I genetic background of both these alleles also occurs alone.

^eOf 28 strains tested, 4 secreted SpeB (1 at near wild-type levels, 3 at reduced levels; 24 lacked detectable SpeB).

^fOf 7 strains tested, 1 secreted SpeB (at reduced levels; 6 lacked detectable SpeB).