Table 2.
Allele No. | Codon | Nucleotide Change | Amino Acid Change | Occurrences, No. | Position in Codon | Strain Collections | SpeB Expression and Activity | Strains Tested for SpeB, No.a |
---|---|---|---|---|---|---|---|---|
1 | Wild-type | None | None | 852 | … | All | Wild-typeb | 9 |
2 | 1 | G3A | M1I | 1 | 3 | OI | Absent | 1 |
3 | 1 | T2A | M1K | 1 | 2 | US | Absent | 1 |
4 | 6 | A16C | T6P | 1 | 1 | OI | Reduced | 1 |
5 | 7 | G19A | V7I | 104 | 1 | OI, US, UK, OP | Reducedc | 56 |
6 | 7, 61 | G19A, T182C | V7I L61P | 1 | 2 | OP | Absent | 1 |
7 | 7, 103 | G19A, T307C | V7I S103Pd | 1 | 1 | OP | Absent | 1 |
8 | 7, 104 | G19A, C311T | V7I T104I | 1 | 2 | OP | Absent | 1 |
9 | 7, 226 | G19A, G677A | V7I R226Qd | 47 | 2 | OI, OP | Absente | 28 |
10 | 7, 235 | G19A, T704C | V7I I235T | 1 | 2 | US | Absent | 1 |
11 | 11 | G33-1 | FS/truncation | 1 | 1 | US | Absent | 1 |
12 | 22 | G65A | C22Y | 8 | 2 | OI, US, OP | Absent | 7 |
13 | 28 | G83A | R28K | 1 | 2 | UK | Absent | 1 |
14 | 28 | A82G | R28G | 1 | 1 | GDR | Absent | 1 |
15 | 29 | C85G | Q29E | 1 | 1 | GDR | Absent | 1 |
16 | 31 | A92G | Y31C | 1 | 2 | US | Absent | 1 |
17 | 33 | C97 + 18 | 6 AA Insertion | 4 | In frame | US | Reduced | 2 |
18 | 33 | C97A | R33S | 1 | 1 | AP | Reduced | 1 |
19 | 34 | T100A | F34I | 1 | 1 | OI | Absent | 1 |
20 | 43 | C128A | Nonsense | 1 | 2 | OI | Absent | 1 |
21 | 55 | T164A | V55D | 1 | 2 | US | Absent | 1 |
22 | 57 | G169A | V57I | 3 | 1 | US | Wild-type | 3 |
23 | 58 | G172A | D58N | 1 | 1 | AP | Absent | 1 |
24 | 62 | T186 + 1 | FS/truncation | 7 | 1 | AP | Absentf | 7 |
25 | 63 | T188C | I63T | 12 | 2 | US | Absent | 2 |
26 | 68 | A204C | K68N | 2 | 3 | US | Wild-type | 1 |
27 | 72 | G214T | Nonsense | 1 | 1 | US | Absent | 1 |
28 | 73 | T218G | F73C | 1 | 2 | US | Absent | 1 |
29 | 75 | G223A | D75N | 1 | 1 | US | Wild-type | 1 |
30 | 76 | G228A | M76I | 1 | 3 | US | Absent | 1 |
31 | 80 | A240C | K80N | 14 | 3 | US, OP | Absent | 2 |
32 | 83 | T248C | F83S | 1 | 2 | OI | Absent | 1 |
33 | 85 | G254A | C85Y | 11 | 2 | OI, US | Reduced | 4 |
34 | 87 | C261 + 1 | FS/truncation | 2 | 1 | US | Absent | 2 |
35 | 90 | G268A | G90D | 1 | 1 | US | Absent | 1 |
36 | 91 | T272C | L91S | 1 | 2 | US | Absent | 1 |
37 | 94 | T281A | I94N | 1 | 2 | OI | Absent | 1 |
38 | 100 | G298 + 20 | FS/truncation | 1 | 2 | US | Absent | 1 |
39 | 103 | T307C | S103P | 5 | 1 | OI, reference | Reduced | 2 |
40 | 103 | C308A | Nonsense | 1 | 2 | US | Absent | 1 |
41 | 105 | A314T | K105M | 1 | 2 | US | Absent | 1 |
42 | 107 | G321T | K107N | 3 | 3 | US | Wild-type | 3 |
43 | 111 | G331A | A111T | 3 | 1 | US | Absent | 2 |
44 | 111 | C332A | A111D | 1 | 2 | UK | Absent | 1 |
45 | 134 | C402-8 | FS/truncation | 1 | 1 | US | Absent | 1 |
46 | 136 | C406A | L136I | 1 | 1 | US | Reduced | 1 |
47 | 140 | G418-1 | FS/truncation | 4 | 2 | OP | Absent | 4 |
48 | 140 | G418 + 6 | 2 AA insertion | 1 | In frame | US | Absent | 1 |
49 | 142 | G425A | Nonsense | 1 | 2 | US | Absent | 1 |
50 | 143 | G428A | S143N | 1 | 2 | OI | Absent | 1 |
51 | 150 | T448A | F150I | 1 | 3 | US | Absent | 1 |
52 | 151 | A451T | N151Y | 3 | 1 | OI | Absent | 2 |
53 | 151 | T453A | N151K | 1 | 3 | US | Absent | 1 |
54 | 152 | A455T | N152I | 1 | 3 | US | Absent | 1 |
55 | 154 | G462A | M154I | 1 | 3 | OI | Absent | 1 |
56 | 173 | C517T | L173F | 1 | 1 | US | Absent | 1 |
57 | 179 | T536A | L179Q | 1 | 2 | US | Absent | 1 |
58 | 180 | G539A | R180K | 1 | 2 | GDR | Absent | 1 |
59 | 184 | T552A | N184K | 2 | 3 | OI | Absent | 2 |
60 | 189 | G567A | M189I | 3 | 3 | OI, US | Absent | 2 |
61 | 196 | T587G | L196W | 2 | 2 | UK | Absent | 2 |
62 | 202 | A605G | E202G | 1 | 2 | US | Absent | 1 |
63 | 206 | C617A | A206D | 1 | 2 | OI | Absent | 1 |
64 | 206 | C617-1 | FS/truncation | 1 | 3 | UK | Absent | 1 |
65 | 220 | G658T | D220Y | 3 | 1 | UK | Absent | 2 |
66 | 222 | G665A | C222Y | 4 | 2 | OI, AP | Absent | 4 |
67 | 224 | T670C | Y224H | 4 | 1 | OI | Absent | 2 |
68 | 226 | G677A | R226Q | 1 | 2 | US | Absent | 1 |
69 | 227 | G680A | C227Y | 1 | 2 | OI | Absent | 1 |
70 | 232 | T696 + 1 | Nonsense | 2 | 1 | OI, UK | Absent | 1 |
71 | 232 | T696-1 | FS/truncation | 6 | 1 | US, GDR | Absent | 2 |
72 | 237 | G710T | G237V | 1 | 2 | OI | Absent | 1 |
73 | 238 | T713C | L238P | 2 | 2 | OP | Absent | 2 |
74 | 245 | G733A | A245T | 2 | 1 | OI, GDR | Absent | 2 |
75 | 245 | G733C | A245P | 2 | 1 | US | Absent | 2 |
76 | 247 | C739A | Nonsense | 4 | 1 | OI | Absent | 2 |
77 | 249 | G746A | C249Y | 2 | 2 | US | Absent | 2 |
78 | 252 | A754T | I252F | 1 | 1 | OI | Absent | 1 |
79 | 252 | C756-14 | FS/truncation | 2 | 1 | US | Absent | 2 |
80 | 256 | T767C | F256S | 1 | 2 | US | Absent | 1 |
81 | 260 | A779C | N260T | 1 | 2 | UK | Wild-type | 1 |
82 | 267 | G801A | M267I | 2 | 3 | OI, US | Absent | 2 |
83 | 268 | T803G | F268C | 1 | 2 | OI | Absent | 1 |
84 | 271 | T811C | Y271H | 1 | 1 | AP | Reduced | 1 |
Abbreviations: AA, amino acid; AP, Alberta pharyngitis strains; GDR, German Democratic Republic invasive strains; OI, Ontario invasive strains; OP, Ontario pharyngitis strains; SpeB, secreted streptococcal cysteine protease B; UK, United Kingdom invasive strains; US, United States Centers for Disease Control and Prevention invasive strains.
Randomly selected invasive strains and all pharyngitis strains with a regulator of protease B gene (ropB) polymorphism were tested for SpeB.
All strains tested secreted high levels of SpeB.
Of 56 strains tested, 48 secreted SpeB (10 at near wild-type levels, 38 at reduced levels; 8 lacked detectable SpeB).
The second single-nucleotide polymorphism occurring in the V7I genetic background of both these alleles also occurs alone.
Of 28 strains tested, 4 secreted SpeB (1 at near wild-type levels, 3 at reduced levels; 24 lacked detectable SpeB).
Of 7 strains tested, 1 secreted SpeB (at reduced levels; 6 lacked detectable SpeB).