TABLE 3.
Fatty acid profiles of isolated LBs from Y. lipolytica wild-type strain W29 and POX-altered mutants MTLY36-2P and MTLY35a
| Fatty acid | % of total fatty acids in strain:
|
||
|---|---|---|---|
| W29 | MTLY36-2P | MTLY35 | |
| C14:0 | 0.6 | NDb | NDb |
| C14:1 | 1.3 | 4.2 | 5.7 |
| C16:0 | 5.5 | 5.2 | 3.4 |
| C16:1 | 16 | 12 | 11 |
| C18:0 | 1.1 | 0.9 | NDb |
| C18:1 | 66 | 70 | 72 |
| C18:2 | 8.7 | 7.3 | 7.3 |
| C20:1 | 0.7 | 1.1 | 1.5 |
| Unsaturated/saturated fatty acid ratio | 13 | 15 | 28 |
| C14:1/C16:1/C18:1 | 2/24/100 | 6/17/100 | 8/15/100 |
a
LBs were isolated from YNBO medium-grown cells at 20 h. Fatty acids in the LBs were converted to methyl esters and analyzed by gas-liquid chromatography.
b
ND, not detected.