Figure 2.
Reduction of α-SMA fibers and cells in TM of Tg-MYOCY437H/+/Sod2+/− mice. (A) Frozen eye sections of wild-type mice were stained with anti-myocilin (left panel) and anti-α-SMA (middle panel) antibodies. Right panel is the merged image of two fluorescent channels and bright field. Scale bar, 100 μm. (B) Whole mouse eye ball was stained with anti-α-SMA antibody and its green fluorescent image was overlaid on the bright-field image. The yellow square indicates a representative region encompassing TM shown in (C). (C) The anterior segments including TM were dissected from 10-month-old littermates and TM tissues in the segments were stained with α-SMA antibodies. At least six independent samples per each genotype were stained. Representative images are shown. Scale bar, 100 μm. (D) The nuclei in the α-SMA-positive area of 10- to 12-month-old mice were counted and represented as the numbers per region. All the values are presented as mean ± SE and analyzed by one-way ANOVA followed by Tukey's multiple comparisons test. **P < 0.01. n shows the number of analyzed mice; TM, trabecular meshwork; CB, ciliary body; SC, Schlemm's canal.