Figure 6.
Nerve fiber layer atrophy and structural change in the ONH of Tg-MYOCY437H/+/Sod2+/− mice. Nerve fibers were labeled by anti-NFH antibodies in the whole-mounted retinas of 10-month-old littermates. 3D image stacks for nerve fiber layer in the ONH and surrounding areas of the retina are shown in the upper row. The corresponding transverse sections stained with antibodies against NFH and TO-PRO-3 are shown in the lower row. Similar results were obtained in three independent experiments. Representative stainings are shown. Scale bar, 100 μm. (B) Quantification of the nerve fiber layer thickness. The measurements were made at the points marked by the arrowheads in the lower set of images as in (A). All the values are presented as mean ± SE. **P < 0.01. n shows the number of analyzed mice.