Figure 8.
Increased expression of a human glaucoma marker, ELAM-1 in TM of Tg-MYOCY437H/+/Sod2+/− mice. Frozen eye sections of 8-month-old littermates were stained with antibodies against ELAM-1. The top panels show the bright-field images of the mouse iridocorneal angle. The middle (bright field) and bottom (ELAM-1 immunofluorescence) panels show the higher magnification for the area surrounded by yellow squares in the top panels. Similar results were obtained in three independent experiments. Representative stainings are shown. Scale bars, 50 μm; TM, trabecular meshwork; CB, ciliary body; SC, Schlemm's canal. (B) Quantification of ELAM-1 fluorescence intensities in the TM areas as in (A). All the values are presented as mean ± SE. *P < 0.05; **P < 0.01. n shows the number of analyzed mice.