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. Author manuscript; available in PMC: 2015 May 29.
Published in final edited form as: J Invest Dermatol. 2014 Apr 2;134(8):2192–2201. doi: 10.1038/jid.2014.165

Figure 4. JMJD3 knock-down influences the expression of HBD-3, S100A7, S100A8 S100A9 and cathelicidin.

Figure 4

Fetal, neonatal and adult KC cultured under proliferating (a) and differentiated (b) conditions were investigated for JMJD3 expression levels using quantitative real-time PCR. Fetal KC were found to express significantly higher levels of JMJD3 as compared to neonatal and adult KC (a,b Mann Whitney test, *p<0.05, **p<0.005). The median and individual data points of four to nine different donors per group are shown; each data point represents the mean of a measurement with three replicates. (c) Western blot analysis showed lower expression of H3K27me3 in fetal KC, as compared to neonatal and adult KC. Western blot of one representative experiment and densitometric analysis of two independent experiments are shown. (d) siRNA mediated knock-down of JMJD3 in fetal KC resulted in significantly lower expression of JMJD3, HBD-3, S100A7, S100A8, S100A9 and cathelicidin. The mean and SEM of two independent experiments with 3 different siRNAs each done in triplicates are depicted; paired t-test, *p<0.05, **p<0.005;