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Induction of protease activity in V. anguillarum M93Sm (squares) and NB10 (circles) cells incubated in LB20 or NSSM. Cells were grown for 16 h in LB20, harvested by centrifugation (10 min at 9,000 × g, 4°C), washed twice in NSS, and resuspended at 2 × 109 CFU/ml in either LB20 (open symbols) or NSSM (solid symbols). Samples were taken at the indicated times, and the cell supernatant was assayed for protease activity.
