FIG. 5.

(A) Induction of protease activity in V. anguillarum M93Sm (squares), NB10 (circles), and M99 (triangles) by conditioned LB20 cell supernatants. V. anguillarum empA mutant strain M99 was grown for 20 h in LB20, and conditioned supernatant was prepared as described in Materials and Methods. V. anguillarum cells were grown for 16 h to 2 × 10⁹ CFU/ml in LB20, prepared as described in the legend to Fig. 1, and resuspended in conditioned LB20 supernatant (solid symbols) or fresh LB20 (open symbols). (B) Conditioned LB20 supernatant was prepared from M99 as described and diluted with fresh LB20. V. anguillarum M93Sm was grown overnight and resuspended at 2 × 10⁹ CFU/ml in the diluted conditioned LB20: 100% (solid squares), 87.5% (solid circles), 75% (solid triangles), 62.5% (solid diamonds), and 0% (open squares).