FIG. 6.
AHL production measured with E. coli JB525 gfp-based (A and B) and E. coli JM109L lux-based (C and D) AHL sensor strains. Ethyl acetate extracts prepared from M93Sm (squares) and NB10 (circles) growing in LB20 (A and C) or NSSM (B and D) were added to gfp-based (which responds to C6-C8 AHLs) and lux-based (which responds to C10-C12 AHLs) sensor strains and observed for stimulation of luminescence or fluorescence, respectively, was measured. The number of CFU per milliliter (solid symbols) was determined at each time point and is indicated in addition to lux or gfp activity (open symbols).