FIG. 7.
Induction of protease activity in V. anguillarum M93Sm and NB10 by ethyl acetate extracts from conditioned LB20 supernatants. Conditioned supernatants from the V. anguillarum empA mutant, M99, were extracted with ethyl acetate. M93Sm (squares) and NB10 (circles) cells were suspended at 2 × 109 CFU/ml in fresh LB20 containing ethyl acetate extract from either M99 (closed symbols) or fresh LB20 (open symbols). As a control, M93 and NB10 cells were suspended in ethyl acetate extract from fresh LB20 (open symbols) and observed for normal induction of protease activity.