FIG. 8.
(A) Induction of protease activity in V. anguillarum by conditioned LB20 supernatants from M01 (luxS mutant). V. anguillarum M93Sm (open symbols) and M01 (closed symbols) were grown for 16 h and resuspended at 2 × 109 CFU/ml in conditioned LB20 supernatant from M01 (triangles), M99 (squares), or fresh LB20 (circles). (B) Induction of protease activity in V. anguillarum M93Sm (squares) and M01 (circles) in Atlantic salmon GI mucus. Cells were grown overnight in LB20, washed twice in NSS, and resuspended at 2 × 109 CFU/ml in 200 μg of mucus protein/ml of NSS (NSSM).