TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype and description | Source or reference |
|---|---|---|
| Strains | ||
| V. anguillarum | ||
| M93Sm | Spontaneous Smr mutant of M93 (serotype J-O-1) | Denkin & Nelson (13) |
| M99 | Smr Cmr; M93Sm empA mutant | This study |
| M01 | Smr Cmr; M93Sm luxS mutant | This study |
| M03 | Smr Cmr; M93Sm rpoS mutant | This study |
| NB10 | Wild type (serotype O1) | Milton et al. (26) |
| NB12 | Cmr; NB10 empA mutant | Milton et al. (26) |
| NB03 | Smr Cmr; NB10 rpoS mutant | This study |
| V. harveyi | ||
| BB120 | Wild type | Bassler et al. (43) |
| BB152 | luxMN (AI-1− AI-2+) | Bassler et al. (43) |
| BB170 | luxN::Tn5 (sensor 1− sensor 2+) | Bassler et al. (43) |
| E. coli | ||
| SM10 | thi thr leu tonA lacY supE recA RP4-2-Tc::Mu::Km (λ pir) | Milton et al. (26) |
| JB525 | Tcr; MT102(pJBA132) | Andersen et al. (2) |
| JM109L | Apr; JM109(pSB1075) | Winson et al. (47) |
| Plasmids | ||
| pNQ705-1 | Cmr; suicide vector with R6K origin | Milton et al. (26) |
| pempA | Cmr Tcr; pSUP202 with 4-kbp empA | Milton et al. (26) |
| pJBA132 | luxR-PluxI-RBSII-gfp (ASV) in pME6031 (detects C6- to C8-AHLs) | Andersen et al. (2) |
| pSB1075 | luxCDABE fused to lasRI′ in pUC18 (detects C10- to C12-AHLs) | Winson et al. (47) |
| pNQEmpA | Cmr; 440-bp empA in pNQ701-1 | This study |
| pNQLuxS | Cmr; 270-bp luxS in pNQ705-1 | This study |
| pNQRpoS | Cmr; 250-bp rpoS in pNQ705-1 | This study |