TABLE 2.
Primers used in this study
| Primer | Sequencea |
|---|---|
| pNQ705-R | 5′-GCGTAACGGCAAAAGCACCGCCGGACATCA-3′ |
| empA-F | 5′-GCTATTCATGTACCGACGCG-3′ |
| empA-R | 5′-CGGAAGGATTTGAAAATGTCGC-3′ |
| empAUSP1 | 5′-GTTATGTGCACTATTAAATGTAC-3′ |
| empADSP1 | 5′-GACGCTCGTTTTGCACTATTTTTC-3′ |
| empAUSP2 | 5′-GATCCATTTTCAATGAGCGAATC-3′ |
| empADSP2 | 5′-GGATTGAGAAATGAAAAAAGTAC-3′ |
| empAP2-1 | 5′-GCTGGTGGTTGATTGGATGTAAG-3′ |
| empAP2-2 | 5′-GGATGTAAGTCGTCAGCGGATGC-3′ |
| empAP2-3 | 5′-GCGGATGCTAATAATAACTCTC-3′ |
| empAP2-4 | 5′-TAATAATAACTCTCTAGGATTGAG-3′ |
| empATXNST | 5′-GAATCATTCGCAGTCACGTC-3′ |
| empA-GSP1 | 5′-GCGTAAGTGCAATACTAA-3′ |
| empA-GSP2 | 5′-GGTGAAGTACTTACCACATCAGAA-3′ |
| 5′RACE-AAP | 5′-GGCCACGCGTCGACTAGTACGGGIIGGGIIGGGIIG-3′ |
| 5′RACE-AP | 5′-GGCCACGCGTCGACTAGTACAAAAAAAAAAAAAAAA-3′ |
| SDempA-F | 5′-GCTAGGAGCTCAAACCATGCAGAGGCG-3′ |
| SDempA-R | 5′-GCTAGTCTAGACCAAAGGTCATGGACG-3′ |
| SDluxS-F2 | 5′-GCTAGGAGCTCCGTATGAATGCACCRGC GG-3′ |
| SDluxS-R | 5′-GCTAGTCTAGAGGCGYACCAATCAAGCT CATG-3′ |
| SDrpoS-F | 5′-GCTAGGAGCTCCGTGGYGATGAAGCSGCWCG-3′ |
| SDrpoS-R | 5′-GCTAGTCTAGACGCAGATAAATGTTCAG CTC-3′ |
a
Restriction sites for SacI (GAGCTC) and XbaI (TCTAGA) are underlined, and mixed bases are in bold (R = A or G, S = G or C, W = T or A, and Y = C or T).