FIG. 1.

Search for revertants by PCR amplification. (A) Locations of primers on the chromosomal DNAs of wt GII-3 and G76; (B) ethidium bromide-stained agarose gel of DNAs amplified with primer pairs AB1-IS4 (lanes 1) and AB1-AB9 (lanes 2). GII-3, DNA from periwinkle plant infected by wt strain GII-3; G76c, DNA from a culture of mutant G76; P4, P5, P7, DNA from periwinkle plants P4, P5, and P7, respectively, infected by mutant G76 at week 11; HP, DNA from a healthy plant; M, 1-kb DNA ladder. Sizes are indicated in base pairs.