TABLE 2.

AEA and 2-AG levels in leptin-treated mHypoE-N41cells and in primary hypothalamic neurons

Endogenous levels of AEA and 2-AG were quantified by LC-atmospheric pressure chemical ionization-MS in mHypoE-N41 cells treated with leptin (100 ng/ml) with or without URB597 (1 μm) or in primary cultures of hypothalamic neurons isolated from C57BL/6 or MAGL null mice. AEA and 2-AG levels are normalized per ml of cell + medium. Each sample contained 1 × 10⁻⁵ and 0.5 × 10⁻⁵ cells/ml for N41 and primary neurons, respectively. Results represent the means ± S.E. of three separate experiments and were compared by using ANOVA followed by the Bonferroni's test.

	AEA	2-AG
	pmol/ml	pmol/ml
mHypoE-N41cells
Untreated	0.15 ± 0.05	2.91 ± 0.55
Leptin	0.20 ± 0.01	3.13 ± 0.005
Leptin+ URB597	0.89 ± 0.04a	2.43 ± 0.31
Primary hypothalamic neurons
Wt
Untreated	0.07 ± 0.01	5.51 ± 0.26
MAGL−/−
Untreated	0.04 ± 0.01	11.23 ± 0.54*
Leptin	0.12 ± 0.03	11.45 ± 0.96*

^a , p > 0.05.