FIGURE 2.

The AcpP and AcpA proteins and the chimeras constructed. The segments from L. lactis AcpA are given in blue, whereas the E. coli AcpP segments are colored puce. The colors plus the letters A and P denote the origins of the sequences (AcpA and AcpP, respectively). The abilities of the proteins to complement growth an acpP deficient strain of E. coli in either the absence or presence of the promiscuous B. subtilis Sfp 4′-phosphopantetheinyl transferase are given in the right-hand columns. The modified serine is the first residue of helix II. w denotes weak growth (approximately one-tenth of wild type), whereas (w) denotes very weak growth (approximately one-twentieth of wild type). e denotes the unexplained erratic growth of these strains.