TABLE 1.
Plasmids used in the work
| Plasmid | Replication origina | Antibiotic selectionb | Promoter: gene | Sources |
|---|---|---|---|---|
| pNRD25 | pUC | Amp | paraBAD: acpP | Ref. 10 |
| pNRD73 | pUC | Amp | paraBAD: acpA | Ref. 4 |
| pKK233–2 | ColE1 | Amp | ptac | Ref. 24 |
| pCY939 | ColE1 | Amp | ptac: acpA | This work |
| pCY941 | ColE1 | Amp | ptac: acpA/acpP II | This work |
| pCY983 | ColE1 | Amp | ptac: acpP/acpA II | This work |
| pCY955 | ColE1 | Amp | ptac: acpP/acpA II VE | This work |
| pCY956 | ColE1 | Amp | ptac: acpP/acpA II VEM | This work |
| pCY957 | ColE1 | Amp | ptac: acpP/acpA II VEMA | This work |
| pCY969 | ColE1 | Amp | ptac: acpP/acpA II F40V | This work |
| pCY970 | ColE1 | Amp | ptac: acpP/acpA II E41Q | This work |
| pCY982 | pUC | Cml | Promoterless acpP | This work |
| pCY983 | ColE1 | Amp | ptac: acpA I + l/acpP II, III, IV | This work |
| pCY985 | ColE1 | Amp | ptac: acpA I/acpP II, III, IV | This work |
| pREP4 | p15A | Kan | Wild type lacI | Qiagen |
| pMS421 | pSC101 | Spc | placIQ lacI | Ref. 2 |
| pCY938 | pUC | Amp | placIQ lacI | This work |
| pNRD136 | RSF1030 | Kan | placIQ lacI:sfp | Ref. 4 |
| pCY947 | RSF1030 | Kan | placIQ lacI:sfp lacIQ | This work |
| pCY948 | RSF1030 | Kan | Δsfp, placIQ lacI | This work |
| pCY765 | p15A | Spc | paraBAD: acpP | This work |
a The pUC origin is that of ColE1 lacking the rop gene.
b The antibiotic abbreviations are: Amp, ampicillin; Kan, kanamycin; Cml, chloramphenicol; and Spc, spectinomycin.