FIGURE 4.

PINK1 is required for parkin-mediated mitochondrial hyperfusion. A, HeLa cells stably expressing YFP-parkin were co-transfected with plasmids encoding PINK1-shRNA and pcDNA-EGFP. Cells were treated with DMSO or CCCP (100 nm, 18 h). Transfected cells were tracked with exogenous EGFP signal (shown in green, cells indicated with green arrows in zoomed in panels) and mitochondria were immunostained using a Tom20 antibody. Scale bar represents 10 μm. B, mitochondrial morphology was assessed in non-transfected and PINK1-shRNA expressing YFP-parkin HeLa cells treated with DMSO or 100 nm CCCP for 18 h. The average ± S.D. was plotted. All statistical significance was assessed using two-way ANOVA analysis with Tukey's test.