FIGURE 8.

α-Synuclein is required for stress-induced mitochondrial fission. A, confocal micrographs of control (cKD) and α-SYNUCLEIN (syn) KD HeLa cells treated with DMSO or 1 μm CCCP for 18 h, as labeled, are shown. Mitochondria were imaged using cytochrome c antibodies (shown in green). Scale bar represents 25 μm. Small squares represent ×5 zoom images. B, mitochondrial morphology was assessed in control (cKD) and syn KD HeLa cells treated with DMSO (−) or 1 μm CCCP for 18 h (+). The average ± S.D. was plotted. The statistical significance was assessed using two-way ANOVA analysis with Tukey's test. C, Western blot of whole cell lysates from control (cKD) and syn KD samples, treated with DMSO control (−) or 1 μm CCCP and immunoblotted with α-synuclein and tubulin, as labeled, are shown.