FIGURE 2.

Effects of mutation of Arg¹⁹⁸ and other amino acids on RNF170 expression. cDNAs encoding wild-type (WT) and mutant RNF170 constructs and vector alone were transfected into HeLa cells and cell lysates were probed as indicated. Erlin2 and β-tubulin served as loading controls. A, lysates were probed with anti-RNF170, which detects both endogenous and exogenous untagged RNF170 constructs (lanes 1–5), or with anti-FLAG, which detects just exogenous FLAG-tagged constructs (lanes 6–10). B, lysates were probed with anti-FLAG and the histogram shows combined quantitated immunoreactivity (mean ± S.E., n ≥ 3).