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. 2015 Apr 14;290(22):13972–13980. doi: 10.1074/jbc.M115.638197

FIGURE 1.

FIGURE 1.

Chronic hyperinsulinemia induces downregulation of the insulin receptor. A, linear regression analysis of liver InsRβ protein level as a function of the logarithm of the circulating insulin level in adult Insr+/− mice. AU, arbitrary units. B, InsRβ protein was quantified by densitometric analysis of the Western blot shown in B, in which the number of each lane corresponds to the same numbered point in A. C, Western blot analysis of InsR signaling in hepatocytes following chronic insulin treatment. Each lane contains equal amounts of protein pooled from three independent experiments. The densitometric quantification of InsR protein relative to Akt is labeled above each lane. pT308, phospho-Thr-308; pS473, phospho-Ser-473; pS9, phospho-Ser-9; pS2448, phospho-Ser-S2448; p, phosphorylated; un-p, unphosphorylated; Gck, glucokinase; pre-Rx, pretreatment.