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. 2015 Apr 11;290(22):14004–14018. doi: 10.1074/jbc.M114.628818

FIGURE 1.

FIGURE 1.

Targeting strategy and confirmation of null allele. A, DNA construct used to target the mouse Wisp1 gene and resulting targeted allele. A, ApaI; B, BglII; RI, EcoRI; K, KpnI; D, DpnI. B, Northern blotting of RNA derived from calvarial cells of 3-day-old mice. C, real-time PCR of mRNA extracted from osteogenic differentiated BMSCs. ***, p < 0.001 versus the wild-type (+/+) sample. D, Western blotting of protein extracted from hind limb bones for probing for Wisp1 (upper panel) Coomassie Brilliant Blue (CBB) staining of the same gel was used to confirm equal loading (lower panel). E, Western blotting of two separate preparations of protein extracted from osteogenic differentiated WT or Wisp1−/− BMSCs while probing for Wisp1.