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. 2015 Apr 11;290(22):14004–14018. doi: 10.1074/jbc.M114.628818

FIGURE 7.

FIGURE 7.

Effect of Wisp1 deficiency on colony-forming efficiency and the osteogenic differentiation of BMSCs. A, representative image of the number of colonies initiated by a single colony-forming unit-fibroblast formed in WT and Wisp1−/− mice. B, enumeration of colonies shown in A. White bar, WT; black bar, Wisp1−/−. C–E, quantification of the relative expression levels of osteogenic differentiation markers measured by real-time PCR in WT and Wisp1−/− BMSCs cultured with osteogenic differentiation medium for 2 weeks: alkaline phosphatase (Alp; C), bone sialoprotein (Bsp; D), and osteocalcin (Ocn; E). F, Alizarin Red S staining of WT and Wisp1−/− BMSCs cultured with osteogenic medium for 1–3 weeks (1W, 2W, and 3W). G, relative levels of Alizarin Red accumulation in cells cultured in the absence (white bars) and presence (black bars) of recombinant WISP1. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus the wild-type (+/+) sample. n.s., not significant.