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. 2015 Apr 23;290(22):14226–14234. doi: 10.1074/jbc.M115.654269

FIGURE 3.

FIGURE 3.

Red IscS also accumulates in wild-type E. coli cells with depletion of intracellular iron. Recombinant IscS was expressed in wild-type E. coli cells grown in LB medium supplemented with 0 (spectrum 1), 0.4 mm 2,2′-dipyridyl (spectrum 2), or 0.4 mm 2,2′-dipyridyl and 0.4 mm Fe(NH4)2(SO4)2 (spectrum 3). Purified IscS proteins (20 μm) were subjected to UV-visible absorption measurements. Inset, a photograph of IscS proteins purified from wild-type E. coli cells grown in LB medium supplemented with 0 (1) or 0.4 mm 2,2′-dipyridyl (2).