Skip to main content
. 2015 May 29;6:368. doi: 10.3389/fpls.2015.00368

FIGURE 2.

FIGURE 2

A26 and A26Δsfp quantification in wheat kernel assay. QPCR quantification of bacterial DNA extracted from wheat grains inoculated with A26 and A26Δsfp as well as un inoculated wheat grains after 5, 10, and 15 days (A) F. graminearum and (B) F. culmorum; (pg bacterial DNA/100 ng plant DNA). Data shown as a means of two experiments; Bars represents SD; Different letters indicate statistically significant differences (P ≤ 0.01) based on the LSD test. (C) PCR analysis for bacterial DNA using 16S A26 primers (identifying both A26 and A26Δsfp) and sfpdel primers (identifying only A26). DNA extract from pure cultures of A26 and A26Δsfp was used as a positive control while DNA extracted from untreated wheat grains was used as a negative control.