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Purification of Photorhabdus sp. strain Az29 proteases
| Purification step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Supernatant | 33.32 | 184,000 | 5,522 | 11 | 100 |
| Ammonium sulfate | 8.71 | 70,000 | 8,037 | 1.46 | 38 |
| DEAE-Sepharose | 2.38 | 67,340 | 28,306 | 5.13 | 37 |
| HiTrap Q-Sepharose PrtA | 0.39 | 13,076 | 33,442 | 6.06 | 7 |
| HiTrap Q-Sepharose PrtS | 1.0 | 22,950 | 22,723 | 4.12 | 12 |
| Mono-Q PrtS | 0.17 | 4,455 | 26,518 | 4.8 | 2 |
