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Bacterial strains and plasmids used in this study
| Strains and plasmids | Characteristics | Reference(s) or source |
|---|---|---|
| Strains | ||
| L. lactis NZ9000 | MG1363 pepN::nisRK | 9, 20a |
| L. lactis NZ9010 | NZ9000 ldh::ery Eryr | 3, 17 |
| L. lactis HWA217 | Reduced PFK activity | 1 |
| Plasmids | ||
| pNZ8148 | pNZ8048 derivative; Cmr; lactococcal cloning and expression vector with nisA promoter upstream of a multi- ple cloning site | 9, 20a |
| pNZmt1D | pNZ8148 carrying L. plantarum mtlD gene | This work |
| pNZ9530 | EryrnisRK | 18 |
